结核杆菌培养基的种类及应用（Types and applications of tubercle bacillus culture medium）.docVIP

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结核杆菌培养基的种类及应用（Types and applications of tubercle bacillus culture medium）.doc

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结核杆菌培养基的种类及应用（Types and applications of tubercle bacillus culture medium）

结核杆菌培养基的种类及应用（Types and applications of tubercle bacillus culture medium） Source: Chinese Paper Download Center [08-02-02 16:55:00] author: Wang Xian, He Shumei, Yuan Li editor: studa20 [Key words] Mycobacterium tuberculosis Since the discovery of Koch in 1882 in order to promote the growth of Mycobacterium tuberculosis, Mycobacterium tuberculosis, shorten training time, to advance the isolation and identification of the purpose of people of Mycobacterium tuberculosis in nutrition, physiological metabolism and artificial culture conducted a series of studies, after more than a century of unremitting efforts, has a more in-depth understanding of Mycobacterium tuberculosis the growth of the law, and the formation of many mature culture method. Although modern molecular biology technology plays an increasingly important role in the research of tuberculosis, but the culture of Mycobacterium tuberculosis in the diagnosis of tuberculosis, tuberculosis epidemiological indicators, classification and identification, drug sensitivity test, drug research still plays an irreplaceable role. In order to understand and select the appropriate and proper culture medium systematically, this paper summarizes the types and applications of the mature medium at home and abroad. 1 Classification according to the characters of culture medium The culture medium of Mycobacterium tuberculosis mainly consists of solid medium, liquid medium, semi fluid medium and solid-liquid two-phase medium, each of which has its own characteristics. 1.1 solid medium is the most commonly used Roche (Lownstein-Jenson, L-J) medium, is one of the most representative, the other is Ogawa Ji (Tatsujiogawa) and Middle brook 7H10 egg medium, 7H11 agar medium etc.. In the solid medium, due to the direct observation of colony morphology and can be distinguished by isolation, therefore, commonly used in clinical specimen identification, preservation of bacteria and anti TB drug sensitivity testing etc, the disadvantage