脂多糖刺激肺血管内巨噬细胞环氧合酶-2表达的意义（Significance of lipopolysaccharide stimulated cyclooxygenase -2 expression in pulmonary vascular macrophages）.docVIP

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脂多糖刺激肺血管内巨噬细胞环氧合酶-2表达的意义（Significance of lipopolysaccharide stimulated cyclooxygenase -2 expression in pulmonary vascular macrophages）.doc

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脂多糖刺激肺血管内巨噬细胞环氧合酶-2表达的意义（Significance of lipopolysaccharide stimulated cyclooxygenase -2 expression in pulmonary vascular macrophages）

脂多糖刺激肺血管内巨噬细胞环氧合酶-2表达的意义（Significance of lipopolysaccharide stimulated cyclooxygenase -2 expression in pulmonary vascular macrophages） Significance of lipopolysaccharide stimulated cyclooxygenase -2 expression in pulmonary vascular macrophages Update Date: 2011-01-18, Wu Weiling, Chen Zhengtang, Cai Enqi, Luo Xianrong, Li Shengliang Abstract: Objective To investigate the effect and significance of lipopolysaccharide (LPS) on cyclooxygenase 2 (COX-2) in pulmonary vascular macrophages (PIM). Improved method of separation, cultivation method of pig PIM, LPS (10 g/ml) stimulation, reverse transcription polymerase chain reaction (RT-PCR) detection of changes in COX-2 mRNA; immunohistochemical staining was used to observe the change of COX-2 protein; radioimmunoassay (RIA) determination of prostaglandin E2 (PGE2) changes in indirect COX activity change. Results after LPS stimulation, PIM COX-2 and mRNA protein expression and PGE2 concentration increased sharply, significantly higher than the control group (P 0.01); COX-2 protein expression is associated with the supernatant of PGE2 was positively (r=0.94, P0.01). Conclusion after LPS stimulation, PIM and COX-2 induce the expression of prostaglandin PGs synthesis and release, which may play an important role in the pathogenesis of acute lung injury (ALI). Keywords: lipopolysaccharides, macrophages, pulmonary intravascular, prostaglandin, lung injury, acute Changes, of, cyclooxygenase-2, in, pulmonary, intravascular, macrophages, stimulated, lipopolysaccharides, by WU, Weiling, CHEN, Zhengtang, CAI, Enqi, et, al. (Cancer, Center, of, PLA, Xingqiao, Hospital, The, Third, Military, Medical, University, Chongqing,, China) Abstract:Objective To study the changes of cyclooxygenase-2 (COX-2) in pulmonary intravascular macrophages (PIMs) stimulated by lipopolysaccharides (LPS). Methods With modification, porcine PIMs were isolated, cultured and stimulated by LPS (10 g/ml). The changes of COX-2 mRNA was detected by reverse Transcr