马齿苋等中药对新分离的痢疾杆菌药物敏抗实验观察(Experimental observation on drug sensitivity of purslane and other traditional Chinese medicine to newly isolated Shigella).docVIP
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马齿苋等中药对新分离的痢疾杆菌药物敏抗实验观察(Experimental observation on drug sensitivity of purslane and other traditional Chinese medicine to newly isolated Shigella)
马齿苋等中药对新分离的痢疾杆菌药物敏抗实验观察(Experimental observation on drug sensitivity of purslane and other traditional Chinese medicine to newly isolated Shigella)
Experimental observation on drug sensitivity of purslane and other traditional Chinese medicine to newly isolated Shigella
Update Date: 2009-05-22 click:
Liu Songtao, Xu Jianhui, Man Guoyu
Key words: Chinese medicine; Shigella; drug sensitivity test
In the past 1996~1997 years, 30 new strains of dysentery bacillus isolated from diarrhea patients in 3 hospitals of our city were selected, and 10 kinds of Chinese herbs were selected for drug and susceptibility test respectively. The results were reported as follows.
1 experimental materials
The experimental strains: 30 strains of Shigella were isolated from stool, direct clinical diarrhea patients, the colony and cell and G staining, consistent with biochemical identification of Shigella, and diagnostic factor serum, type (determined by serum agglutination diagnosis of Chengdu Institute of biological products and health epidemic prevention station of Shandong Province Supply within the period of validity, use).
Medium: Double broth (used as experimental strain liquid culturing and experimental dilution), EmB agar (Shanghai medical laboratory supply, according to the product that is prepared to use).
Chinese medicinal liquor production: selected Coptis, paeonol, tangerine peel, Yin Chen, purslane, Sophora, hawthorn, honeysuckle, rhubarb, grass, sieved to miscellaneous, each weighing 50g. Add water right amount, boil 1 ~ 1.5 hours, filter to remove slag, then decoct, steam, condense to 50ml (that is, as 100% of Chinese medicine sensitive liquid).
2 experimental method
Experimental bacteria: respectively from each experimental strains were detected bacteria inoculation slant, double tube broth (per tube broth 10ml, 37 DEG C) were incubated for 12 to 18 hours, for drug sensitivity test of bacteria. The bacterial fluid can be diluted for testing according to the test.
Test liqui
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