高中生物教材实验相关知识梳理（High school biology textbook experiment related knowledge carding）.docVIP

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高中生物教材实验相关知识梳理（High school biology textbook experiment related knowledge carding）.doc

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高中生物教材实验相关知识梳理（High school biology textbook experiment related knowledge carding）

高中生物教材实验相关知识梳理（High school biology textbook experiment related knowledge carding） High school biology textbook experiment related knowledge carding I. The use of common instruments, reagents or drugs 1, NaOH: pH used to absorb CO2 or change the solution 2, Ca (OH) 2: identification of CO2 3, CaCl2: increase the permeability of bacterial cell wall 4, HCl: dissociation (15%) or change the pH of the solution 5, NaHCO3: provide CO2, as acid-base buffer 6 acid base buffer (Na2CO3/NaHCO3, Na2HPO4/NaH2PO4): used to regulate the solution pH 7, NaCl: preparation of physiological saline (0.9%) or for the extraction of DNA (0.14M or 2M) 8 agar: a carrier or medium for hormones or other substances, used for hormone transfer or culture 9, alcohol: used for disinfection (75%), purify DNA (95%), leaf decolorization, preparation of dissociation liquid (95% of cold alcohol) and wash off floating color (50%) 10. Sucrose: preparation of sucrose solution, determine the concentration of plant cell fluid, or observe the separation and restoration of the wall 11, two aniline: for the identification of DNA (boiling water bath, blue) 12, methyl green: detection of DNA, green 13, piroxicam: detection of RNA, red 15, Fehling Reagent (a solution of 0.1g/mL NaOH, 0.05g/mLCuSO4 / class B liquid) reagent: identification of soluble reducing sugar (boiling water bath, brick red precipitate) 16, for the identification of the protein: biuret reagent (purple) 17, Sultan III dye (Orange) and Sultan IV (red) for fat liquor identification 18, iodine liquid: used to identify starch (blue) 19, gentian violet solution or acetic acid magenta: basic dyes for chromosome staining, the former is deep blue, the latter is red 20, improved phenol fuchsin: detection of chromosome, red 21, the green B: the detection of mitochondria, specificity, so that mitochondria stained blue-green 22, potassium dichromate solution: detection of alcohol, gray green 23, bromine blue blue water solution: detection of CO2, from blue