高效液相色谱法测定六味地黄胶囊中丹皮酚含量（Determination of Paeonol in six kinds of Rehmannia capsule by HPLC）.docVIP

高效液相色谱法测定六味地黄胶囊中丹皮酚含量（Determination of Paeonol in six kinds of Rehmannia capsule by HPLC） Determination of Paeonol in six kinds of Rehmannia capsule by HPLC Update Date: 2010-12-22 Click: Zhang Zhongchun, Guan Yumin, Wang Jian, Wang Guanlian Abstract: the content of Paeonol in six flavor Rehmannia capsule was determined by HPLC, the mobile phase was 70% methanol, the detection wavelength was 274 nm, and the average recovery was 99.48%. The method is simple, accurate and reproducible. Keywords Six Ingredient Rehmannia capsule; paeonol; HPLC; Determination, of, Paeonol, in, Liuweidihuang, Capsule, by, HPLC Zhang, Zhongchun, Guan, Yumin, Wang, Jian, et, Al Shandong, University, of, Traditional, Chinese, Medicine, Jinan 250012 Abstract HPLC method has been used for the determination of Paeonol in Liuweidihuang capsule with methanol as mobile phase and detecting 70% wavelength of 274 nm. The average recovery is 99.48%. The method is simple and accurate. Key, words, Liuweidihuang, capsule; Paeonol; HPLC The six is the traditional Chinese medicine Liuweidihuang capsule Six Ingredient Rehmannia Pill dosage form reform varieties, has the effect of nourishing yin and tonifying kidney, composed of Radix rehmanniae, Cortex Moutan, dogwood, Poria, Alisma, yam, contained in the ministerial standard eighth copies. The content of Paeonol in the sample was determined by steam distillation UV spectrophotometry. We found in the experiment that the method is tedious, time-consuming and inaccurate, instead of the HPLC method, the method is simple, fast, accurate and reproducible. 1 instrument and reagent SP-8800 high performance liquid chromatography, Spectra type 200 UV detector, Chromfet integrator, standard 10 L injection ring. Paeonol control substance (purchased in China Pharmaceutical and biological products testing institute, HPLC normalization method, purity 99.628%), water as double distilled water, methanol as chromatographic purity. The sample provided by the Jinan factory of