miR-223在胰腺癌细胞中表达及对增殖、凋亡及细胞周期的影响.pdfVIP

中文摘要 凋亡和细胞周期的影响，探索其作用机制。 方法l以实时定量PCR(re扯time 此后利用生物信息学方法和双荧光素酶报告基因检测系统寻找miR-223的靶基因；最后 990细胞， 采用CCK-8法检测细胞增殖，AnnexinV／PI双染法检测分析细胞凋亡，流式细胞术检 测分析细胞周期变化。 等预测软件中汇总分析并筛选出候选靶基因FOXOla，双荧光素酶报告基因检测结果显 示FOXOla组与阳性对照组相比相对荧光值降低，具有统计学差异(P0．05o在SWl990 期细胞比例增加，G1期细胞比例下降，G2／M期未见规律性改变；本次实验通过上调或 下调111iR-223表达未观察到对胰腺癌细胞凋亡的影响。， 察到其对胰腺癌细胞凋亡的影响。 关键词：miR-223，胰腺癌，靶基因，细胞增殖，凋亡，细胞周期 Abstract in celllinesand the miR-223 cancer objective：Toanalyze expressionpancreatic investigate ofmiR-223on cell andcell ofthe CanCea impact proliferation，cellapoptosiscycle pancreatic as cellswell弱themechanismsofaction． Methods：WemiR-223 in cancereelllines real．timePC：R． analyze expressionpancreatic by we a to Subsequently employedbio—computationalapproachidemi矽miR-223targetgene followedwithdualluciferase wetransfectedT3M4 reporterassayvalidation．Finally cancelcells withHlil0223mimicsandtransfectedSWl990 cancercells pancreatic pancreatic withmi】R-223inhibitorto the ofmiR．223onthe functions．Cell investigateimpact biological WaS detectedwithCCK．8：Cell was withAnnexinV／PI proliferation apoptosisanalyzed distributionwas withflow assay：Cellcycle analyzed c吼oInetry． Results：miR-223wasdetectedinall8 eelllines our expression pancreatic pos