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- 2017-09-03 发布于江苏
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人重组Ⅱ,17蛋台的表达及其生物学特性的初步分析 英文摘要
the hum8n】1广17 and
1.Cl佃ingcDNA∞oding Ihll-lengthnon-signalpep“de
fhgment
弧c cDNA forhum飘Ⅱ广17
publi妣dsequ锄ce w觞tlsed衙即翻丘cpimerd髂i弘.
of
hum锄m-17 ofhum觚几-17
如ll-IellgtIl g锄e触dn∞一si印alpeptide矗a鲫ent g%e
PHA·activatcd b100dT删ls.髓朗
w勰枷plifiedthrou曲RT-PCR触nhum锄p喇pheml
PCEP4Vectors锄dIL广17 double化stricti∞
digcstcdPQE3.0柚d aquipedg胁esby
method.Ne嫩worlc to tll部e afI盯
enzymedjg略t w鹊usinglagasel谵ate pmducb
w嚣v硝fied thevector
f∞oV盯y。Thedefj唰pl渤{d byDNA∞qq翱ce粕a】ysis粕d
naIIled were订柚sf0咖edintoTOPl0
PCEP钔L—17 strainof
PQE3.帆L一17髓d
Eschcfichiacolifor certmed dcfived
bydigest锄dPcRtlle
pmliferati∞.ARef
recombiIlant w舔饥msfonnediIltoM15咖inofEscherichiac0Hfor
PQE3.Opl嬲mid
fhrnlcr
exp托ssi∞of如Sion峭in.
of
andcharacteri数tion fusion in
2.Expression,purina啦on PQE3.O,I】■17 protein
E.coIi
was iIIlmbrotll.Aseri髓of蛐油0ds
M15既p嘲si吼straillpropagated Oow
of toilldu∞soluble
tempe馏tllreinduction,shonImilldu吐i吼time,etc)w酃tfied
儿一】7脚is觚i∞p彤teinbut白iled.Fusi∞proteinw拈mainlyexpmssed笛inclu如n
indicaI谢tlIattllemost 5
body.Dyn枷ics
study
IPTG
h叫r inductionatlmmol/L.Thecell tll曲
S1uf
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