基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书.docx

基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书供应商：上海乔羽生物有限公司基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书Kit performance1 sensitivity: the minimum concentration is less than 1 standard. Linearity of dilution. Sample linear regression and the expected concentration correlation coefficient R value is 0.990.2 specificity: no reaction with other cytokines.3 repeatability: the variation coefficient of the plate and the plate are all less than 10%.Result judgment and analysis1, the instrument value: Yu Bo 450nm ELISA od read the hole on the instrument2, to the OD value as the ordinate, y, corresponding HLA-B27 standard concentration as the abscissa, x, do the corresponding curve, HLA-B27 content in the sample can be according to its OD value by standard curve conversion out corresponding concentration.3, value range: 0-8.0IU/ml4, sensitivity: 0.01 IU/ml基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书Kit composition:Sealing film: 2 (48) / 2 (96)Specifications: 1 copiesSealing bag: 1Standard: 2700ng / L 0.5 * 0.5ml * 1 bottles, 1 bottles are stored in 2-8ELISA package is board: 1 X 48 x 961 2-8 stored inSample dilution: 3ml * 1 ml * 1 bottles of 6 bottles are stored in 2-8Color agent: Liquid 3ml * 1 ml * 6 bottles of 1 bottles in 2-8Color reagent B Liquid 3ml * 1 ml * 1 bottle 6 bottles stored in 2-8Termination solution: 3ml * 6ml * 1 bottles and 1 bottles are stored in 2-8Concentrated laundry liquid: (2 * 20) * 1 bottles (20ml * 30) * 1 bottles stored in 2-8elisa试剂盒价格，elisa试剂盒说明书，elisa检测试剂盒基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书ELISA技术原理：以免疫学反应为基础，将抗原、抗体的特异性反应与酶对底物的高效催化作用相结合起来.1. 抗原或抗体的固相化及抗原或抗体的酶标记。2. 结合在固相载体表面的抗原或抗体仍保持其免疫学活性。3. 酶标记的抗原或抗体既保留其免疫学活性，又保留酶的活性。4. 受检标本与固相载体表面的抗原或抗体起反应。再加入酶标记 的抗原或抗体，也通过反 应而结合在固相载体上。5. 此时固相上的酶量与标本中受检物质的量呈一定的比例。6. 加入酶反应的底物后，底物被酶催化成为有色产物，产物的量 与标本中受检物质的量直 接相关，故可根据呈色的深浅进行定性或定量分析。测定方法具有很高的敏感度(pg-ng/ml 水 平)，并且重复性好。基质金属蛋白酶1ELISA试剂盒，兔ELISA试剂盒说明书Operational matters needing attention:The reagent should label storage instructions, return to room temperature before use. After thinning of