about the effects of estradiol on the proliferation of tca8113（关于tca8113的雌二醇对扩散的影响）.docVIP

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about the effects of estradiol on the proliferation of tca8113（关于tca8113的雌二醇对扩散的影响）.doc

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about the effects of estradiol on the proliferation of tca8113（关于tca8113的雌二醇对扩散的影响）

About the effects of estradiol on the proliferation of Tca8113 Papers to write network: Author: Liu Yali Chi Sumin Zhu Yunlong Xue Caifang [Keywords:], estradiol Keywords: estradiol; carcinoma, squamous cell; the tongue tumor; Splinter Cell Abstract: Objective To observe the impact of estradiol (estradiol, E2 Tca8113 cell lines cultured human tongue squamous cell carcinoma cell proliferation. E2 cultured in vitro tongue squamous cell carcinoma cells using tetrazolium salt (MTT) than color test and 3 H-TdR incorporation was measured The MTT reaction the A490nm values #8203;#8203;and the amount of 3 H-TdR incorporation and cell cycle was determined by flow cytometry. Results of different concentrations of E2 (10-8 to 10-6 mol ?L -1 can significantly increase the proliferation of human tongue squamous cell carcinoma Tca8113 cells A490nm value of the rate of change of the MTT (107.5 + -2.6%, respectively, (113.1 + -3.1% and (120.0 + -3.7% (P lt;0.01 cpm the rate of change of the value, respectively (108.2 + -4.0% (116.7 + -4.2% (124.8 + -3.2% (P lt;0.01), and the existence of dose effect .10-6 mol · L-1 β-E2 make human tongue carcinoma cells into S phase and G2 phase of the cell ratio rose to 21.1% from 16.3% and 7.2% and 16.6% of the proportion of cells in G1 phase decreased from 76.5% to 62.3% (P lt;0.01, thereby increased DNA synthesis of cells, promoting their proliferation Tca8113 cells promote the proliferation of E2 on human tongue squamous cell carcinoma is Tamoxifen blocked. conclusion E2 significantly promote the proliferation of cultured human tongue squamous cell carcinoma cells in vitro. Keywords: estradiol; carcinoma, squamous cell; tongue neo-plasms; cell division Abstract: AIM To study the influence of estradiol on the proliferation, DNA synthesis and cell cycle of cultured human tongue squamous cell carcinoma Tca8113cells.METHODS MTT method, 3 H-TdR incorporation and cell cycle analysis were used to examine the changes in proliferatio