肝癌转移特征性microRNAs的筛选和功能鉴定.pdfVIP

目 录 中文 摘 要 ··············································································… … 1 英 文 摘 要 ··············································································… …3 前 言 ····················································································… …6 第 一部 分 肝 癌 转 移 特 征 性 mi cro RN As 的筛 选 材 料 与 方 法 ···········································································… …9 结 果 ····················································································… … 14 讨 论 ····················································································… … 18 第 二部 分 m icr0RNLA6 12 在 肝 癌 转 移 过程 中 的功 能验 证 材 料 与方 法 ···········································································… …20 结 果 ····················································································… … 36 讨 论 ····················································································… …55 参 考 文 献 ··············································································… …5S 全 文 总 结 ··············································································… …66 创 新 点 ·················································································… …66 不足 与后续研 究 ·····································································……66 文 献 综 述 ··············································································… …68 附录 ····················································································… …75 致 谢 ····················································································… … 78 论 文 声 明 ··············································································… …80 复旦 大 学硕 士 学位 论 文 中文 摘 要 背 景 与 目的 : mi CroRNAs (m iRNA s)是 一种 短 链 非编 码 小 RNA ，通 过 与其 靶 基 因 mRNA 3 ’UTR 区结合 发挥 转 录 后抑 制 作 用 ，进 而 调节 细 胞凋 亡 、分化 、代 谢 、 运 动 以及 参 与肿瘤 的发生和 发 展 。近 年 来 有研 究证