Genistein对人瘢痕疙瘩成纤维细胞增殖及侵袭作用的影响.docVIP

Genistein对人瘢痕疙瘩成纤维细胞增殖及侵袭作用的影响 曹 川，戴 霞，李世荣，李 丹，张 谊，冯 智，陈艳清 (第三军医大学：西南医院整形外科，重庆 400038) 提 要: 目的 观察57,4’-三羟基异黄酮(Genistein)对体外培养的人瘢痕疙瘩成纤维细胞生长及侵袭作用的影响，探讨其抗纤维化作用的。方法 以5、50、100μmol/L浓度Genistein处理体外培养的人瘢痕疙瘩成纤维细胞，MTT检测细胞增殖H3-脯氨酸掺入法检测细胞胶原合成，Transwell小室趋化运动模型检测细胞侵袭。结果 Genistein瘢痕疙瘩成纤维细胞的增殖及胶原合成，细胞的侵袭能力下降结论 Genistein具有体外抗瘢痕疙瘩纤维化与侵袭的作用，可成为治疗瘢痕及纤维化疾病的有效药物。 5,7,4’-三羟基异黄酮；瘢痕疙瘩；成纤维细胞；增殖；侵袭The effects of Genistein on the proliferation and invasion of human keloid fibroblasts Cao Chuan, Dai Xia, Li Shi-rong, Li Dan, Zhang Yi, Feng Zhi, Chen Yan-qing. (Department of Plastic Surgery, Southwestern Hospital，Third Military Medical University, Chongqing 400038) AbstractObjective To observe the effects of Genistein on the proliferation and invasion of the cultured human keloid fibroblasts and explore the mechanism of its anti-fibrosis. Methods The human keloid fibroblasts were cultured and stimulated with 25, 50, 100μmol/L Genistein respectively. The cells’ proliferation was measured with MTT staining method. The collagen synthesis was assessed by H3-proline incorporation method. The invasion of cells was detected in Transwell chemotaxis chamber model. Results Compared with control groups, Genistein can markedly inhibit the proliferation and collagen synthesis of the cultured keloid fibroblasts and the inhibiting effects were increased with the drug’s dosage. Genistein can also weaken the invasion of keloid fibroblasts. Conclusion Genistein has the ability of anti-fibrosis as well as inhibiting of cells’ invasion in vitro, which may become an effective drug for pathological scar or fibrosis disease. Key words: Genistein; keloid; fibroblast; proliferation; invasion 瘢痕疙瘩(eloid)是一种胶原等细胞基质过度沉积为特征的皮肤纤维化疾病具有侵袭性生长的特性。目前发病机制，也无满意的治疗药物手术切除极易复发5，7，4’－三羟基异黄酮(Genistein)是一种特异性酪氨酸蛋白激酶(tyrosine protein kinase，TPK)抑制剂，对多种肿瘤和纤维化具有作用［5］本实验Genistein对瘢痕疙瘩增殖和作用的影响，为临床应用Genistein提供依据。苏州YJ-875型超净工作台、美国SHEL- LAB1815TC型二氧化碳培养箱、美国Beckman LS26500液体闪烁计数仪、美国BIO-RAD 550型酶标仪Transwell小室(滤膜为μm孔径的无聚碳酸脂聚乙烯吡咯烷酮多孔滤膜)和Matrige