a portable luminometer with a disposable electrochemiluminescent biosensor for lactate determination一个便携式光度计对乳酸测定一次性electrochemiluminescent生物传感器.pdfVIP

下载本文档

0
0
约6.62万字
约 17页
2017-09-05 发布于上海
举报
版权申诉

a portable luminometer with a disposable electrochemiluminescent biosensor for lactate determination一个便携式光度计对乳酸测定一次性electrochemiluminescent生物传感器.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

a portable luminometer with a disposable electrochemiluminescent biosensor for lactate determination一个便携式光度计对乳酸测定一次性electrochemiluminescent生物传感器

Sensors 2009, 9, 7694-7710; doi:10.3390/ OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination Antonio Martínez-Olmos 1, Julio Ballesta-Claver 2, Alberto J. Palma 1, Maria del Carmen Valencia-Mirón 2 and Luis Fermin Capitán-Vallvey 2,* 1 ECsens, Department of Electronics and Computer Technology, Granada, Spain; E-Mails: amartinez@ugr.es (A.M.O.); ajpalma@ugr.es (A.J.P.) 2 ECsens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, Spain; E-Mails: juliosci@ugr.es (J.B.C.); cvmiron@ugr.es (M.C.V.M.) * Author to whom correspondence should be addressed; E-Mail: lcapitan@ugr.es; Tel.: +034-958-248-436; Fax: +034-958-243-328. Received: 16 July 2009; in revised form: 8 September 2009 / Accepted: 17 September 2009 / Published: 28 September 2009 Abstract: A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition and reaction conditions have been optimized to obtain adequate sensitivity. The luminometer is based on a large silicon photodiode as detector and includes a programmable poten