action of chitosan against xanthomonas pathogenic bacteria isolated from euphorbia pulcherrima壳聚糖对植物病原菌的作用从大戟属植物分离pulcherrima.pdfVIP

Molecules 2012, 17, 7028-7041; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima Yanli Wang 1,2, Liping Li 3, Bin Li 1,*, Guoxing Wu 4, Qiaomei Tang 1, Muhammad Ibrahim 1, 1 1 2 Hongye Li , Guanlin Xie and Guochang Sun 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China 2 State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Institute of Plant Protection Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 3 Institute of Bioinformatics, Zhejiang University, Hangzhou 310058, China 4 College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China * Author to whom correspondence should be addressed; E-Mail: libin0571@; Tel.: +86-571-8898-2412; Fax: +86-571-8640-4225. Received: 25 April 2012; in revised form: 17 May 2012 / Accepted: 22 May 2012 / Published: 7 June 2012 Abstract: The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve Xanthomonas strains recovered from Euphorbia pulcherrima . Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three selected strains was increased by both chitosans. However, the release