hplc method for simultaneous determination of the the dream tuoling film melatonin and vitamin b6 content（高效液相色谱法同时测定的梦想到达电影褪黑激素和维生素b6的内容）.docVIP

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hplc method for simultaneous determination of the the dream tuoling film melatonin and vitamin b6 content（高效液相色谱法同时测定的梦想到达电影褪黑激素和维生素b6的内容）.doc

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hplc method for simultaneous determination of the the dream tuoling film melatonin and vitamin b6 content（高效液相色谱法同时测定的梦想到达电影褪黑激素和维生素b6的内容）

HPLC method for simultaneous determination of the the dream Tuoling film melatonin and vitamin B6 content [Keywords] melatonin Abstract: The purpose of using high-performance liquid chromatography and determination in dream Tuoling tablets of melatonin and vitamin B6 content column Dumas Diamonsil C18 column, mobile phase: methanol-water (60:40, detection wavelength: 278 nm. result in the establishment of the chromatographic conditions, melatonin and vitamin B6 can be completely separated. melatonin 0.066 to 0.33 μg vitamin B6 0.024 ~ 0.122 μg range and peak area showed a good linear relationship, r respectively. 0.999 8,0.999 9, the recoveries were 102.1%, 101.9%. to conclusion this Act precision, accurate and reliable results. Keywords:: HPLC, the dream Tuoling tablets, melatonin, vitamin B6 Camel Bells dream piece is the Company’s products, mainly composed of ingredients such as melatonin and vitamin B6 and quality standards of the company only to determine the content of melatonin, not to determine the content of vitamin B6 on melatonin and vitamin B6 analysis methods have been reported in the literature [1,2], but at the same time the determination of both methods have not been reported. this article using the HPLC method for simultaneous determination dream Tuoling film melatonin and vitamin B6 content, easy to operate, good precision accurate and reliable results can better control the quality of the finished product. 1 Instruments and reagents Shimadzu LC-10AT pump, SPD-10A UV detector, Zhejiang University, N2000 workstation. The dream Tuoling chip-based company’s products, melatonin standard (American SIGMA produced), vitamin B6 (by the normalization method content gt; 99%), methanol (HPLC grade), and water (double distilled water). Experimental Methods and Results 2.1 Chromatographic conditions Dumas, Diamonsil C18 column, mobile phase: methanol-water (60:40; detection wavelength: 278 nm, flow rate: 1.0 ml / min, column te