fetal dna in maternal blood by fluorescence quantitative pcr assay and labor relations（胎儿dna在母体血液的荧光定量pcr检测和劳动关系）.docVIP

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2017-09-06 发布于浙江
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fetal dna in maternal blood by fluorescence quantitative pcr assay and labor relations（胎儿dna在母体血液的荧光定量pcr检测和劳动关系）.doc

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fetal dna in maternal blood by fluorescence quantitative pcr assay and labor relations（胎儿dna在母体血液的荧光定量pcr检测和劳动关系）

Fetal DNA in maternal blood by fluorescence quantitative PCR assay and labor relations [Abstract] Objective To study the late pregnancy and labor in maternal fetal DNA content and its clinical significance. Methods use real-time quantitative PCR techniques were used to detect pregnancy from 28 to 42 weeks and labor when the fetal DNA in maternal plasma content and compare. pregnancy results 28 to 31 weeks and 32-35 weeks of pregnancy at gestational week 36 to 42 and perinatal mother plasma, the average content of fetal DNA in comparison differences there are significant (the F = 308.19, q = 11.59 ~ 40.33, P lt;0.01) Conclusion As the pregnancy progresses, fetal DNA content increases in the maternal blood may be one of the incentive for giving birth. [Keywords:] pregnant women, fetus; DNA; polymerase chain reaction [ABSTRACT] ObjectiveTo study the changes of content of fetal DNA in maternal plasma in late pregnancy and in labor, and explore their significance.MethodsThe content of fetal DNA in maternal plasma in 28-42 weeks of gestation and parturient period were detected by using real time quantitative PCR technique.ResultsThe differences of mean content of fetal DNA in maternal plasma among 28-31 week, 32-35 week, and 36-42  week gestation and parturient period were significant (F = 308.19, q = 11.59-40.33, P lt;0.01). ConclusionWith the progress of duration of pregnancy, the fetal DNA in maternal blood increases, which is, most likely, one of the inducements leading to delivery. [KEY WORDS] Pregnant; Women fetus; DNA; Polymerase chain reaction The results showed that fetal DNA in maternal plasma, and found that as the pregnancy progresses, fetal DNA content will be a corresponding increase in [1], but the increased content of fetal DNA in maternal blood lead to labor the occurrence of it have a close relationship with labor and childbirth reported less. This study was designed quantitative PCR technology detected in maternal fetal DNA content