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of in were and for
ABCEIGene
bank,threetargetsequencesdesignedsynthesized
wore
constructionofSiRNAvector.After insertedinto
annealing,oligonucleotides
carriedredfluorescent recombinant
vector,which
pSIREN—DNR protein.The plasmid
andABCEl·SiRNA-Nwereidentified
ABCEl一SiI℃NA一1,ABCEl一SiRNA-2 by
restrictionendonucleaseandDNA too.
sequencing
Smallcell cancelcell cultured研Ⅱl1640culture
lung 1ino(NCI—H446)WaS
solution 10%fetusbovine 37℃锄d FUGENE-HD
containing serous,at 5%C02.Using
and
pEGFP
transfected smallcell can嘣cellline.48hoursafter
were intohuman
-Ct lung
blotand todetect of
transfection,we嗷Westem the懿pression
immunohistochc缸tistry
theABCEl inthetransfectedcells.The
protein
cellsWaS MTTTranswdl mark
ofthe
ability assayedby qlIba啪andexperiment.
WaSusedto whethertheinteffermceVect0哐canindue..
Flow detect
cytometry
ABCElWaS
ofthesmall cell.Meanwhilethe of
apoptosis lung(rancor expression
detectedthe andWesternblotinsmallcell CanCeltissue,
by immunohistochemistry lung
metastasis
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