competitive dna transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells竞争dna通过电穿孔转染配方对人类脂肪干细胞和间充质干细胞.pdfVIP

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competitive dna transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells竞争dna通过电穿孔转染配方对人类脂肪干细胞和间充质干细胞.pdf

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competitive dna transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells竞争dna通过电穿孔转染配方对人类脂肪干细胞和间充质干细胞

Flanagan et al. Biological Procedures Online 2012, 14:7 /content/14/1/7 Biological Procedures Online RESEARCH Open Access Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells 1 2 2 3 4 3,5* Michael Flanagan , Jeffrey M Gimble , Gang Yu , Xueqing Xia , Bruce A Bunnell and Shulin Li Abstract Background: Adipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use. Results: We developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method that we had developed previously. This method was designed to determine the optimal formulation for nucleofection that was capable of meeting or surpassing the established commercial buffer (Amaxa), in particular for murine adipose stem cells. By using this same buffer, we determined that the same formulation yields optimal transfection efficiency in human mesenchymal stem cells. Conclusions: Our findings suggest that transfection efficiency in human stem cells can be boosted with proper formulation. Keywords: Electroporation, Formulation, Stem cells, Transfection, Cell therapy Background DNA in the process of nucleofection [10]. Although Cell-based therapies have great potential for the treat- nucleofection is an effective form of nonviral transfec- ment of genetic disorders as well as currently incurable ti