complementary genetic and genomic approaches help characterize the linkage group i seed protein qtl in soybean互补基因和基因组的方法有助于描述我在大豆种子蛋白质qtl连锁群.pdfVIP

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complementary genetic and genomic approaches help characterize the linkage group i seed protein qtl in soybean互补基因和基因组的方法有助于描述我在大豆种子蛋白质qtl连锁群.pdf

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complementary genetic and genomic approaches help characterize the linkage group i seed protein qtl in soybean互补基因和基因组的方法有助于描述我在大豆种子蛋白质qtl连锁群

Bolon et al. BMC Plant Biology 2010, 10:41 /1471-2229/10/41 RESEARCH ARTICLE Open Access Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean 1† 2† 3 3 4 5 Yung-Tsi Bolon , Bindu Joseph , Steven B Cannon , Michelle A Graham , Brian W Diers , Andrew D Farmer , Gregory D May5 6 7 8 3 8 , Gary J Muehlbauer , James E Specht , Zheng Jin Tu , Nathan Weeks , Wayne W Xu , 3 1,6* Randy C Shoemaker , Carroll P Vance Abstract Background: The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content. Results: A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix® Soy GeneChip and high-throughput Illumina® whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region. Conclusions: This st