培养根霉 自制优质甜酒曲（To cultivate high-quality sweet homemade Rhizopus Koji）.docVIP

培养根霉 自制优质甜酒曲（To cultivate high-quality sweet homemade Rhizopus Koji） Cultivation and observation of Rhizopus Objective to understand the morphological characteristics of Rhizopus and to understand the culture method of rhizopus. Before the experiment, what is the root of Rhizopus? What is the difference between its spores and the way the spores of Penicillium and Aspergillus are produced? Under what conditions does it grow? Materials Steamed Buns appliances or bread, concentrated wine powder; microscope slides and coverslips, dropper, tweezers, dissecting needle, absorbent paper, spray cans, plastic bags or Petri dishes, needle or inoculating loop, thermostat, alcohol lamp, three foot. Asbestos, cotton, aseptic inoculation box, beaker, flask. Methods Rhizopus was cultivated with steamed bread or bread step 1. take a Steamed Buns or bread, one or two cm thick, exposed in the air one or two hours, and then placed in a small plastic bag, with spray wet, put the bag. In a dark place. If the temperature is over 20 DEG C, white villous surface after two or three days Steamed Buns even with the wall of the plastic bags have a loose crumb President Steamed Buns, spread a little bit in the white fluff on the black ash, like ash size, this is the colony of rhizopus. 2. to observe the hyphae and rhizoids (Figure) With the anatomical needle from the steamed bread, pick out some black spots Rhizopus, as far as possible without or with steamed bread crumbs. Place in the water slides of the slides, carefully spread out, but do not pull the hyphae together. The cover on the coverslip, under the microscope can see no diaphragm Rhizopus mycelium (see photo). To contact with the four mycelium spread, Steamed Buns or matrix, like the root length in the process, but no complex structure like seed plant root, so called rhizoids. Contact two rhizoids called hyphae stolon. Observation of 3. sporangia found erect clumps of hyphae from rhizoid, at the top of them having sporangia. The hypha