a reliable method for the selection of exploitable melanoma archival paraffin embedded tissues for transcript biomarker profiling一个可靠的方法的选择利用黑色素瘤档案石蜡包埋组织生物标志物分析记录.pdfVIP

A Reliable Method for the Selection of Exploitable Melanoma Archival Paraffin Embedded Tissues for Transcript Biomarker Profiling 1,2. 3. 1 4 Celeste Lebbe , Mickael Guedj , Nicole Basset-Seguin , Marie Pierre Podgorniak , Suzanne Menashi5, Anne Janin2,6,7, Samia Mourah4,8* ´ ˆ ´ 1 Departement de Dermatologie, hopital saint Louis Paris, Paris, France, 2 Universite Paris 7- Denis Diderot, Paris, France, 3 Ligue Nationale Contre le Cancer, Cartes ´ ˆ ´ ´ d’Identite des Tumeurs program, Paris, France, 4 AP-HP, Hopital Saint-Louis, Laboratoire de Pharmacologie-Genetique, Paris, France, 5 CNRS, EAC 7149, Laboratoire CRRET, ´ ´ ˆ Universite Paris 12, Creteil, France, 6 Inserm, U728, Paris, France, 7 AP-HP, Hopital Saint-Louis, Laboratoire de Pathologie, Paris, France, 8 Inserm, UMR-S 940, Paris, France Abstract The source tissue for biomarkers mRNA expression profiling of tumors has traditionally been fresh-frozen tissue. The adaptation of formalin-fixed, paraffin-embedded (FFPE) tissues for routine mRNA profiling would however be invaluable in view of their abundance and the clinical information related to them. However, their use in the clinic remains a challenge due to the poor quality of RNA extracted from such tissues. Here, we developed a method for the selection of melanoma archival paraffin-embedded tissues that can be reliably used for transcript biomarker profiling. For that, we used qRT-PCR to conduct a comparative study in matched pairs of frozen and FFPE mel