a novel method for culturing of leptothrix sp. strain oums1 in natural conditions纤毛菌属的一种新颖的方法,培养sp。应变oums1自然条件.pdfVIP

Minerals 2012, 2, 118-128; doi:10.3390/min2020118 OPEN ACCESS minerals ISSN 2075-163X /journal/minerals/ Article A Novel Method for Culturing of Leptothrix sp. Strain OUMS1 in Natural Conditions 1 1 1 2 Tomoko Suzuki , Hiromichi Ishihara , Mitsuaki Furutani , Tomonori Shiraishi , Hitoshi Kunoh 1 and Jun Takada 1,* 1 Department of Material Chemistry, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan; E-Mails: suzukito@cc.okayama-u.ac.jp (T.S.); hishihara@cc.okayama-u.ac.jp (H.I.); dns421306@s.okayama-u.ac.jp (M.F.); hkunoh@cc.okayama-u.ac.jp (H.K.) 2 Laboratory of Plant Pathology, Graduate School of Environmental Science, Okayama University, Okayama 700-8530, Japan; E-Mail: tomoshir@cc.okayama-u.ac.jp * Author to whom correspondence should be addressed; E-Mail: jtakada@cc.okayama-u.ac.jp; Tel.: +81-86-251-8107; Fax: +81-86-251-8087. Received: 8 February 2012; in revised form: 23 March 2012 / Accepted: 15 May 2012 / Published: 23 May 2012 Abstract: Although some strains of Leptothrix spp. isolated from aquatic environments have been characterized by culturing them in laboratory conditions, they often show morphological and chemical features distinct from those found in natural environments. To resolve this discrepancy, a novel cultivation method was devised for culturing such strains in natural groundwater. Leptothrix sp. strain OUMS1 was pre-cultured i