原创力文档a novel in vitro method for the detection and characterization of photosensitizers一种新型体外检测方法以及敏化的表征.pdfVIP
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a novel in vitro method for the detection and characterization of photosensitizers一种新型体外检测方法以及敏化的表征
A Novel In Vitro Method for the Detection and
Characterization of Photosensitizers
Nadine Karschuk, Yeliz Tepe, Silke Gerlach, Wolfgang Pape, Horst Wenck, Robert Schmucker, Klaus-
Peter Wittern, Andreas Schepky*, Hendrik Reuter
Beiersdorf AG, Research and Development, Hamburg, Germany
Abstract
Photoactivation and binding of photoactive chemicals to proteins is a known prerequisite for the formation of
immunogenic photoantigens and the induction of photoallergy. The intensive use of products and the availability of new
chemicals, along with an increasing exposure to sun light contribute to the risk of photosensitizing adverse reactions.
Dendritic cells (DC) play a pivotal role in the induction of allergic contact dermatitis. Human peripheral blood monocyte
derived dendritic cells (PBMDC) were thus perceived as an obvious choice for the development of a novel in vitro
photosensitization assay using the modulation of cell surface protein expression in response to photosensitizing agents. In
this new protocol, known chemicals with photosensitizing, allergenic or non-allergenic potential were pre-incubated with
PBMDCs prior to UVA irradiation (1 J/cm2). Following a 48 h incubation, the expression of the cell surface molecules CD86,
HLA-DR and CD83 was measured by flow cytometry. All tested photosensitizers induced a significant and dose-dependent
increase of CD86 expression after irradiation compared to non-irradiated controls. Moreover, the phototoxicity of the
chemicals could also be determined. In contrast, (i) CD86 expression was not affected by the chosen irradiation conditions,
(ii) increased CD86 expression induced by allergens was independent of irradiation and (iii) no PBMDC activation was
observed with the non-allergenic control. The assay propose
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