a retinoic acid responsive hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field一个视黄酸反应hoxa3转基因表达的胚胎咽内胚层,心脏神经嵴和第二心脏领域的子域名.pdfVIP
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a retinoic acid responsive hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field一个视黄酸反应hoxa3转基因表达的胚胎咽内胚层,心脏神经嵴和第二心脏领域的子域名
A Retinoic Acid Responsive Hoxa3 Transgene Expressed
in Embryonic Pharyngeal Endoderm, Cardiac Neural
Crest and a Subdomain of the Second Heart Field
1¤ 1 2,3 4 ´ 2,3
Nata Y. S.-G. Diman , Sophie Remacle , Nicolas Bertrand , Jacques J. Picard , Stephane Zaffran *,
´ 1*
Rene Rezsohazy
´
1 Molecular and Cellular Animal Embryology group, Life Sciences Institute, Universite catholique de Louvain, Louvain-la-Neuve, Belgium, 2 UMR910, Aix-Marseille
´
University, Marseille, France, 3 Medical Genetics and Functional Genomics, Inserm UMR_S910, Marseille, France, 4 Faculty of Medicine, Universite catholique de Louvain,
Brussels, Belgium
Abstract
A transgenic mouse line harbouring a b-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3
was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of
transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing
heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second
heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm.
Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression o
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