a simple method for dna extraction from mature date palm leaves impact of sand grinding and composition of lysis？buffer一个简单的方法从成熟日期棕榈叶中提取dna的影响砂磨和溶解的组成缓冲区.pdfVIP

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a simple method for dna extraction from mature date palm leaves impact of sand grinding and composition of lysis？buffer一个简单的方法从成熟日期棕榈叶中提取dna的影响砂磨和溶解的组成缓冲区.pdf

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a simple method for dna extraction from mature date palm leaves impact of sand grinding and composition of lysis？buffer一个简单的方法从成熟日期棕榈叶中提取dna的影响砂磨和溶解的组成缓冲区

Int. J. Mol. Sci. 2010, 11, 3149-3157; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis Buffer Ibrahim A. Arif, Mohammad A. Bakir, Haseeb A. Khan *, Anis Ahamed, Ahmad H. Al Farhan, Ali A. Al Homaidan, Mohammad Al Sadoon, Ali H. Bahkali and Mohammad Shobrak Molecular Fingerprinting and Biodiversity Unit, Prince Sultan Research Chair for Environment and Wildlife, College of Sciences, King Saud University, Riyadh, Saudi Arabia; E-Mails: iaarif@; (I.A.A.); mabakir@ (M.A.B.); nanisahamed@ (A.A.); alfarhan@.sa (A.H.A.F.); homaidan@.sa (A.A.A.H.); msadoon@.sa (M.A.S.); abahkali@.sa (A.H.B.); mshobrak@ (M.S.) * Author to whom correspondence should be addressed; E-Mail: haseeb@.sa; Tel.: +966-1-4674-712. Received: 19 July 2010; in revised form: 8 August 2010 / Accepted: 10 August 2010 / Published: 8 September 2010 Abstract: Molecular marker techniques have been widely used for cultivar identification of inbred date palms (Phoenix dactylifera L.; Arecaceae) and biodiversity conservation. Isolation of highly pure DNA is the prerequisite for PCR amplification and subsequent use such as DNA fingerprinting and sequencing of genes that have recently been developed for barcoding. To avoid problems related to the preservation and use of liquid nitrogen, we examined sterile sand for grindi