a three-component gene expression system and its application for inducible flavonoid overproduction in transgenic arabidopsis thaliana三分量的基因表达系统及其应用在转基因拟南芥诱导类黄酮生产过剩.pdfVIP

A Three-Component Gene Expression System and Its Application for Inducible Flavonoid Overproduction in Transgenic Arabidopsis thaliana 1 1 1 2 3 3 Yue Feng , Cong-Mei Cao , Meenu Vikram , Sunghun Park , Hye Jin Kim , Jong Chan Hong , Luis 1 1 Cisneros-Zevallos , Hisashi Koiwa * 1Vegetable and Fruit Improvement Center, Department of Horticultural Science, Texas AM University, College Station, Texas, United States of America, 2 Department of Horticulture, Forestry and Recreation Sources, Kansas State University, Manhattan, Kansas, United States of America, 3 BK21 Program, Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Graduate School of Gyeongsang National University, Jinju, Korea Abstract Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD p hosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of A nthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgen