a review on the effects of supercritical carbon dioxide on enzyme activity综述了超临界二氧化碳对酶活性的影响.pdfVIP
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a review on the effects of supercritical carbon dioxide on enzyme activity综述了超临界二氧化碳对酶活性的影响
Int. J. Mol. Sci. 2010, 11, 233-253; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Review
A Review on the Effects of Supercritical Carbon Dioxide on
Enzyme Activity
Zdeněk Wimmer 1 and Marie Zarevúcka 2,*
1 Institute of Experimental Botany AS CR, Isotope Laboratory, Vídeňská 1083, 142 20 Prague
4–Krč, Czech Republic; E-Mail: wimmer@biomed.cas.cz
2 Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo náměstí 2, 166 10 Prague
6–Dejvice, Czech Republic
* Author to whom correspondence should be addressed; E-Mail: zarevucka@uochb.cas.cz;
Tel.: +420-220-183-281; Fax: +420-220-183-582.
Received: 23 November 2009; in revised form: 7 January 2010 / Accepted: 9 January 2010 /
Published: 19 January 2010
Abstract: Different types of enzymes such as lipases, several phosphatases,
dehydrogenases, oxidases, amylases and others are well suited for the reactions in SC-CO .
2
The stability and the activity of enzymes exposed to carbon dioxide under high pressure
depend on enzyme species, water content in the solution and on the pressure and
temperature of the reaction system. The three-dimensional structure of enzymes may be
significantly altered under extreme conditions, causing their denaturation and co
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