a tissue-specific approach to the analysis of metabolic changes in caenorhabditis elegans的修复方法在秀丽隐杆线虫代谢变化的分析.pdfVIP

A Tissue-Specific Approach to the Analysis of Metabolic Changes in Caenorhabditis elegans ¨ 1 . ´ 2,3. 3 1 4 Jurgen Hench * , Ivana Bratic Hench , Claire Pujol , Sabine Ipsen , Susanne Brodesser , Arnaud 5 1 1 ´2,3 Mourier , Markus Tolnay , Stephan Frank , Aleksandra Trifunovic 1 Department of Neuropathology, Institute of Pathology, University Hospital Basel, Basel, Switzerland, 2 Department of Laboratory Medicine, Division of Metabolic Diseases, Karolinska Institutet, Stockholm, Sweden, 3 Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Institute for Genetics, University of Cologne, Cologne, Germany, 4 Lipidomics Core Facility, CECAD and Institute for Medical Microbiology, Immunology, and Hygiene, Cologne, Germany, 5 Max- Planck-Institut for Biology of Ageing, Cologne, Germany Abstract The majority of metabolic principles are evolutionarily conserved from nematodes to humans. Caenorhabditis elegans has widely accelerated the discovery of new genes important to maintain organismic metabolic homeostasis. Various methods exist to assess the metabolic state in worms, yet they often require large animal numbers and tend to be performed as bulk analyses of whole worm homogenates, thereby largely precluding a detailed studies of metabolic changes in specific worm tissues. Here, we have adapted well-established histochemical methods for the use on C. elegans fresh frozen sections and demonstrate their validity for analyses of morphological and metabolic changes on tissue level in wild type and various mutant strains. We show how the worm presents on hematoxylin and eosin (HE) stained sections a