acmnpv core gene ac109 is required for budded virion transport to the nucleus and for occlusion of viral progenyacmnpv核心基因ac109需要发了芽的病毒粒子运输核和闭塞的病毒后代.pdfVIP

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acmnpv core gene ac109 is required for budded virion transport to the nucleus and for occlusion of viral progenyacmnpv核心基因ac109需要发了芽的病毒粒子运输核和闭塞的病毒后代.pdf

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acmnpv core gene ac109 is required for budded virion transport to the nucleus and for occlusion of viral progenyacmnpv核心基因ac109需要发了芽的病毒粒子运输核和闭塞的病毒后代

AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny 1 2 1 ´ ´ 1 1 Victoria Alfonso , Guillermo A. Maroniche , Sol R. Reca , Marıa Gabriela Lopez , Mariana del Vas , Oscar Taboga1* ´ ´ ´ 1 Instituto de Biotecnologıa, CICVyA, Instituto Nacional de Tecnologıa Agropecuaria (IB-INTA), Hurlingham, Buenos Aires, Argentina, 2 Instituto de Microbiologıa y ´ ´ ´ Zoologıa Agrıcola, CICVyA, Instituto Nacional de Tecnologıa Agropecuaria (IMyZA-INTA), Hurlingham, Argentina Abstract The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This defect could be repaired by complementing ac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nuclei infected with ac109 knockout viruses. However, electron microscopy demonstrated that these occlusion bodies were empty. Altogether, these results indicate that ac109 is required for infectivity of both phenotypes of virus. ´ Citation: Alfonso V, Maroniche GA, Reca SR, Lopez MG, del Vas M, et al. (2012) Ac MNPV Core Gene ac109 Is Required for Budded Viri