a three-way comparison of tuberculin skin testing, quantiferon-tb gold and t-spot.tb in children结核菌素皮肤试验的三方比较,quantiferon-tb黄金和t-spot。.pdfVIP
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a three-way comparison of tuberculin skin testing, quantiferon-tb gold and t-spot.tb in children结核菌素皮肤试验的三方比较,quantiferon-tb黄金和t-spot。
A Three-Way Comparison of Tuberculin Skin Testing,
QuantiFERON-TB Gold and T-SPOT.TB in Children
1,2,3. 1,2,3. 4 1,2,3 1,2,3
Tom G. Connell , Nicole Ritz , Georgia A. Paxton , Jim P. Buttery , Nigel Curtis *, Sarath C.
Ranganathan1,2,5
1 Department of Paediatrics, University of Melbourne, Royal Children’s Hospital Melbourne, Parkville, Victoria, Australia, 2 Murdoch Children’s Research Institute, Royal
Children’s Hospital Melbourne, Parkville, Victoria, Australia, 3 Infectious Diseases Unit, Department of General Medicine, Royal Children’s Hospital Melbourne, Parkville,
Victoria, Australia, 4 Immigrant Health Service, Department of General Medicine, Royal Children’s Hospital Melbourne, Parkville, Victoria, Australia, 5 Department of
Thoracic Medicine Royal Children’s Hospital Melbourne, Parkville, Victoria, Australia
Abstract
Background: There are limited data comparing the performance of the two commercially available interferon gamma (IFN-
c) release assays (IGRAs) for the diagnosis of tuberculosis (TB) in children. We compared QuantiFERON-TB gold In Tube (QFT-
IT), T-SPOT.TB and the tuberculin skin test (TST) in children at risk for latent TB infection or TB disease.
Methods and Findings: The results of both IGRAs were compared with diagnosis assigned by TST-based criteria and assessed in
relation to TB contact history. Results from the TST and at least one assay were available for 96 of 100 children. Agreement
between QFT-IT and T-SPOT.TB was high (93% agreement, k = 0.83). QFT-IT and T-SPOT.TB tests were positive in 8 (89%) and 9
(100%) children with suspected active TB disease. There was moderate agreement between TST and either QFT-IT (75%,
k = 0.50) or T-SPOT.TB (75%, k =
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