adventures in the enormous a 1.8 million clone bac library for the 21.7 gb genome of loblolly pine冒险在巨大的180万年克隆21.7 gb基因组bac文库的火炬松.pdfVIP

Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine 1,2 1 1 1,2 Zenaida V. Magbanua *, Seval Ozkan , Benjamin D. Bartlett , Philippe Chouvarine , Christopher A. 3 4 4,5 6 1,2 Saski , Aaron Liston , Richard C. Cronn , C. Dana Nelson , Daniel G. Peterson 1 Mississippi Genome Exploration Laboratory and Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, Mississippi, United States of America, 2 Life Sciences and Biotechnology Institute and Institute for Digital Biology, Mississippi State University, Mississippi State, Mississippi, United States of America, 3 Clemson University Genomics Institute, Clemson University, Clemson, South Carolina, United States of America, 4 Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, United States of America, 5 Pacific Northwest Research Station, United States Forest Service, Corvallis, Oregon, United States of America, 6 Southern Institute of Forest Genetics, United States Forest Service, Saucier, Mississippi, United States of America Abstract Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in the U.S. and a cornerstone species in southeastern forests. However, genomics research on LP and other conifers has lagged behind studies on flowering plants due, in part, to the large size of conifer genomes. As a means to accelerate conifer genome research, we constructed a BAC library for the LP genotype 7-56. The LP BAC library consists of 1,824,768 individu