Survivin基因反义寡核苷酸与大肠癌血管形成的试验研究.docVIP

Survivin基因反义寡核苷酸与大肠癌血管形成的试验研究 李瑞，黄宗海，姚航，厉周，李强（南方医科大学珠江医院普通外科，广州 510282） 摘 要：目的 研究Survivin反义寡核苷酸对人大肠癌细胞裸鼠移植的瘤细胞凋亡及血管形成的影响。方法 建立裸鼠人大肠癌模型，然后将成瘤后的裸小鼠分为空白对照组，脂质体转染对照组，脂质体SODN对照组和脂质体ASODN组，治疗后测定瘤重并进行比较；将瘤组织进行HE染色，检测Survivin在瘤细胞内的表达；用SP法染色检测微血管密度(MVD)，用TUNEL法检测凋亡细胞，并探讨Survivin ASODN与MVD、AI之间的关系。结果 ASODN实验组在第8日后测定最终瘤重，与各对照组在统计学上均有显著性差异（p0.05）。常规病理下ASODN实验组可见肿瘤细胞生长受抑制，肿瘤组织切片中可见片状坏死区，而且这些区域可见大量炎性细胞浸润。空白对照组，脂质体对照组，脂质体SODN对照组MVD分别为19.57±3.37、21.94±4.41、20.49±3.91, ASODN实验组MVD为11.30±2.79，差异显著（P0.05）。空白对照组，脂质体对照组，脂质体SODN对照组AI分别为3.53±1.89、3.86±2.14、4.47±2.30，ASODN实验组AI为28.45±2.76，ASODN实验组AI与空白对照组，脂质体对照组，脂质体SODN对照组AI相比，差异显著（P0.05）。结论 Survivin基因反义寡核苷酸可显著抑制血管内皮生长因子的表达，导致内皮细胞凋亡和毛细血管迅速退化，从而达到抑制肿瘤生长的目的。 关键词：Survivin基因；反义寡核苷酸；大肠癌模型；微血管密度 Study on the effect of survivin ASODN to the vasiformation of colorectal cancer cell LI Rui, HUANG Zong-hai, YAO Hang, LI Zhou, LI Qiang（Department of General Surgery，Zhujiang Hospital，South Medical University，Guangzhou 510282, China） Abstract: AIM To study on the effect of survivin ASODN to the apoptosis of colorectal cancer cell and vasiformation in nude mice. METHODS To build the model of colorectal cancer in nude mice,and then we divided into four groups: control group, Lipofectamine group, Lipofectamine+SODN group and Lipofectamine+ASODN group.We compared the final weight of tumor and calculated the suppression rate of tumor. Hematoxylin and eosin stain of tumor tissues were performed for histological examination .We detected the expression of survivin and microvessel density (MVD) of tumor and the apoptosis of tumor cell. RESULTS The results showed that the final weight of tumor had conspicuous difference between Lipofectamine+ASODN group with other groups (P0.05). The pathological change showed that the growth of tumor was inhibited. The tissue section of tumor showed lamellar zone of necrosis and massive inflammatory cell infiltrate.The microvessel density (MVD) of tumor showed after treatment:control group (19.57±3.37); Lipofectamine group (2