quantitative expression profile of distinct functional regions in the adult mouse brain不同功能区域的定量表达谱成年老鼠的大脑.pdfVIP

Quantitative Expression Profile of Distinct Functional Regions in the Adult Mouse Brain 1. 2,3. 1,2. 3 1 Takeya Kasukawa , Koh-hei Masumoto , Itoshi Nikaido , Mamoru Nagano , Kenichiro D. Uno , 2,4 5 3 1,2,4,6,7 Kaori Tsujino , Carina Hanashima , Yasufumi Shigeyoshi *, Hiroki R. Ueda * 1 Functional Genomics Unit, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan, 2 Laboratory for Systems Biology, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan, 3 Department of Anatomy and Neurobiology, Kinki University School of Medicine, Osaka, Japan, 4 Graduate School of Science, Osaka University, Osaka, Japan, 5 Laboratory for Neocortical Development, RIKEN Center for Developmental Biology, Hyogo, Japan, 6 Department of Mathematics, Graduate School of Science, Kyoto University, Kyoto, Japan, 7 Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Kobe, Hyogo, Japan Abstract The adult mammalian brain is composed of distinct regions with specialized roles including regulation of circadian clocks, feeding, sleep/awake, and seasonal rhythms. To find quantitative differences of expression among such various brain regions, we conducted the BrainStars (B*) project, in which we profiled the genome-wide expression of ,50 small brain regions, including sensory centers, and centers for motion, time, memory, fear, and feeding. To avoid confounds from temporal differences in gene expression, we sampled each region every 4 hours for 24 hours, and pooled the samples for DNA-microarray assays. Therefore