quantitative histological validation of diffusion mri fiber orientation distributions in the rat brain扩散磁共振成像纤维取向分布的定量组织学验证在老鼠大脑.pdfVIP

Quantitative Histological Validation of Diffusion MRI Fiber Orientation Distributions in the Rat Brain 1. 2. 3 1 3 Trygve B. Leergaard , Nathan S. White , Alex de Crespigny , Ingeborg Bolstad , Helen D’Arceuil , 1 4 Jan G. Bjaalie , Anders M. Dale * 1 Centre for Molecular Biology and Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway, 2 Department of Cognitive Sciences, University of California San Diego, San Diego, California, United States of America, 3 A.A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, 4 Departments of Neurosciences and Radiology, University of California San Diego, San Diego, California, United States of America Abstract Diffusion MRI (dMRI) is widely used to measure microstructural features of brain white matter, but commonly used dMRI measures have limited capacity to resolve the orientation structure of complex fiber architectures. While several promising new approaches have been proposed, direct quantitative validation of these methods against relevant histological architectures remains missing. In this study, we quantitatively compare neuronal fiber orientation distributions (FODs) derived from ex vivo dMRI data against histological measurements of rat brain myeloarchitecture using manual recordings of individual myelin stained fiber orientations. We show that accurate FOD estimates can be obtained from dMRI data, even in regions with complex architectures of crossing fibers with an intrinsic orientation error of approximately 5–6 degrees in these regions. The reported f