reciprocal tripartite interactions between the aedes aegypti midgut microbiota, innate immune system and dengue virus influences vector competence倒数三方之间的相互作用的埃及伊蚊中肠微生物群,先天免疫系统和登革热病毒影响矢量能力.pdfVIP

Reciprocal Tripartite Interactions between the Aedes aegypti Midgut Microbiota, Innate Immune System and Dengue Virus Influences Vector Competence 1 1¤ 2 2 2 Jose Luis Ramirez , Jayme Souza-Neto , Rolando Torres Cosme , Jose Rovira , Alma Ortiz , Juan M. 2 1 Pascale , George Dimopoulos * 1 W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America, 2 Gorgas Memorial Institute for Health Research, Panama City, Panama Abstract Dengue virus is one of the most important arboviral pathogens and the causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. It is transmitted between humans by the mosquitoes Aedes aegypti and Aedes albopictus, and at least 2.5 billion people are at daily risk of infection. During their lifecycle, mosquitoes are exposed to a variety of microbes, some of which are needed for their successful development into adulthood. However, recent studies have suggested that the adult mosquito’s midgut microflora is critical in influencing the transmission of human pathogens. In this study we assessed the reciprocal interactions between the mosquito’s midgut microbiota and dengue virus infection that are, to a large extent, mediated by the mosquito’s innate immune system. We observed a marked decrease in susceptibility to dengue virus infection when mosquitoes harbored certain field-derived bacterial isolates in their midgut. Transcript abundance analysis of selected antimicrobial peptide genes suggested that the mosquito’s microbiota elicits a basal immune act