原创力文档rectification of the water permeability in cos-7 cells at 22, 10 and 0°c整流cos-7透水性的细胞在22日10 0°c.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
rectification of the water permeability in cos-7 cells at 22, 10 and 0°c整流cos-7透水性的细胞在22日10 0°c
Rectification of the Water Permeability in COS-7 Cells at
22, 10 and 06C
Diana B. Peckys1,3*, F. W. Kleinhans2,3, Peter Mazur3
1 Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America, 2 Department of Physics,
Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana, United States of America, 3 Department of Biochemistry, and Cellular and Molecular Biology,
University of Tennessee, Knoxville, Tennessee, United States of America
Abstract
The osmotic and permeability parameters of a cell membrane are essential physico-chemical properties of a cell and
particularly important with respect to cell volume changes and the regulation thereof. Here, we report the hydraulic
conductivity, L , the non-osmotic volume, V , and the Arrhenius activation energy, E , of mammalian COS-7 cells. The ratio
p b a
of V to the isotonic cell volume, V , was 0.29. E , the activation energy required for the permeation of water through the
b c iso a
cell membrane, was 10,700, and 12,000 cal/mol under hyper- and hypotonic conditions, respectively. Average values for Lp
were calculated from swell/shrink curves by using an integrated equation for L . The curves represented the volume
p
changes of 358 individually measured cells, placed into solutions of nonpermeating solutes of 157 or 602 mOsm/kg (at 0, 10
or 22uC) and imaged over time. Lp estimates for all six combinations of osmolality and temperature were calculated,
resulting in values of 0.11, 0.21,
您可能关注的文档
- rapamycin regulates the expression and activity of krüppel-like transcription factor 2 in human umbilical vein endothelial cells雷帕霉素调节kruppel-like转录因子的表达和活动2人类脐静脉内皮细胞.pdf
- rapamycin response in tumorigenic and non-tumorigenic hepatic cell lines雷帕霉素的反应在肿瘤发生的和非致瘤性肝细胞系.pdf
- rapamycin inhibits proliferation of hemangioma endothelial cells by reducing hif-1-dependent expression of vegf雷帕霉素抑制扩散血管瘤内皮细胞减少hif-1-dependent vegf的表达.pdf
- rapid acoustic survey for biodiversity appraisal生物多样性快速声学调查评估.pdf
- rapid accumulation of virulent rift valley fever virus in mice from an attenuated virus carrying a single nucleotide substitution in the m rna快速积累毒性裂谷热从一个减毒病毒携带病毒在小鼠单核苷酸替换的m rna.pdf
- range expansion drives dispersal evolution in an equatorial three-species symbiosis范围扩张驱动散布在赤道三种共生进化.pdf
- rapid and high-throughput pan-orthopoxvirus detection and identification using pcr and mass spectrometry快速、高通量pan-orthopoxvirus探测和识别使用pcr和质谱分析.pdf
- rapid and accurate multiple testing correction and power estimation for millions of correlated markers快速和准确的多个测试校正和功率估计为数以百万计的相关标记.pdf
- rapid and long-lasting increase in sites for synapse assembly during late-phase potentiation in rat hippocampal neurons快速和持久的增加网站在后期阶段势差在鼠海马神经元突触组装.pdf
- rapid and sensitive detection of rotavirus molecular signatures using surface enhanced raman spectroscopy快速、敏感的检测轮状病毒分子签名使用表面增强拉曼光谱.pdf
- recursive cluster elimination based support vector machine for disease state prediction using resting state functional and effective brain connectivity疾病状态递归消除集群基于支持向量机预测使用大脑静息状态的功能和有效的连接.pdf
- recruitment of rad51 and rad52 to short telomeres triggers a mec1-mediated hypersensitivity to double-stranded dna breaks in senescent budding yeast招聘rad51和端粒短rad52触发mec1-mediated过敏症在衰老出芽酵母dna双链断裂.pdf
- red and processed meat and colorectal cancer incidence meta-analysis of prospective studies红肉和加工肉类和结直肠癌发病率前瞻性研究的荟萃分析.pdf
- recurrent network models for perfect temporal integration of fluctuating correlated inputs周期性波动相关的完美时间集成网络模型输入.pdf
- recurrent recruitment manoeuvres improve lung mechanics and minimize lung injury during mechanical ventilation of healthy mice复发性招聘演习提高肺力学和最小化在机械通气肺损伤健康的小鼠.pdf
- red blood cell fatty acid patterns and acute coronary syndrome红细胞脂肪酸模式和急性冠脉综合征.pdf
- red queen dynamics with non-standard fitness interactions红桃皇后动力学与非标准健身交互.pdf
- recycling energy to restore impaired ankle function during human walking循环能源在人类步行恢复踝关节功能受损.pdf
- red wine polyphenols prevent metabolic and cardiovascular alterations associated with obesity in zucker fatty rats (fafa)红酒多酚预防代谢和心血管变化与肥胖有关zucker肥老鼠(fafa).pdf
- recurrent chromosome 16p13.1 duplications are a risk factor for aortic dissections复发性染色体16 p13.1重复主动脉剥离的危险因素.pdf
文档评论（0）