recruitment of rad51 and rad52 to short telomeres triggers a mec1-mediated hypersensitivity to double-stranded dna breaks in senescent budding yeast招聘rad51和端粒短rad52触发mec1-mediated过敏症在衰老出芽酵母dna双链断裂.pdfVIP

Recruitment of Rad51 and Rad52 to Short Telomeres Triggers a Mec1-Mediated Hypersensitivity to Double- Stranded DNA Breaks in Senescent Budding Yeast Yi-Hsuan Lin, Chia-Ching Chang, Chui-Wei Wong, Shu-Chun Teng* Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan Abstract Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of telomerase. In both mammalian tumor and yeast cells that lack telomerase, telomeres are maintained by an alternative recombination mechanism. Here we demonstrated that the budding yeast Saccharomyces cerevisiae type I survivors derived from telomerase-deficient cells were hypersensitive to DNA damaging agents. Assays to track telomere lengths and drug sensitivity of telomerase-deficient cells from spore colonies to survivors suggested a correlation between telomere shortening and bleomycin sensitivity. Our genetic studies demonstrated that this sensitivity depends on Mec1, which signals checkpoint activation, leading to prolonged cell-cycle arrest in senescent budding yeasts. Moreover, we also observed that when cells equipped with short telomeres, recruitments of homologous recombination proteins, Rad51 and Rad52, were reduced at an HO-endonuclease-catalyzed double-strand break (DSB), while their associations were increased at chromosome ends. These results suggested that the sensitive phenotype may be attributed to the sequestration of repair proteins to compromised telomeres, thus limiting the repair capacity at bona fide DSB sites. Citation: Lin Y-H, Chang C-C, Wong C-W, Teng S-C (2009) Recruitment of Rad51 and Rad52 to Short Telomeres Triggers a Mec1-Mediated Hypersensitivity to Double-Stranded DNA Breaks in Senescent Budding Yeast.