原创力文档recruitment of oct4 protein to uv-damaged chromatin in embryonic stem cells招聘oct4蛋白质uv-damaged染色质在胚胎干细胞.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
recruitment of oct4 protein to uv-damaged chromatin in embryonic stem cells招聘oct4蛋白质uv-damaged染色质在胚胎干细胞
Recruitment of Oct4 Protein to UV-Damaged Chromatin
in Embryonic Stem Cells
ˇ
´ ´ ´ ˇ ´ ´ ˇ ´
Eva Bartova*, Gabriela Sustackova, Lenka Stixova, Stanislav Kozubek, Sona Legartova, Veronika
´ ´
Foltankova
Department of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Abstract
Background: Oct4 is a specific marker of embryonic stem cell (ESC) pluripotency. However, little is known regarding how
Oct4 responds to DNA damage. Here, we investigated whether Oct4 recognizes damaged chromatin in mouse ESCs stably
expressing GFP-Oct4. These experiments should contribute to the knowledge of how ESC genomic integrity is maintained,
which is crucial for potential application of human ESCs in regenerative medicine.
Methodology/Principal Findings: We used time-lapse confocal microscopy, microirradiation by UV laser (355 nm),
induction of DNA lesions by specific agents, and GFP technology to study the Oct4 response to DNA damage. We found
that Oct4 accumulates in UV-damaged regions immediately after irradiation in an adenosine triphosphate-dependent
manner. Intriguingly, this event was not accompanied by pronounced Nanog and c-MYC recruitment to the UV-damaged
sites. The accumulation of Oct4 to UV-damaged chromatin occurred simultaneously with H3K9 deacetylation and H2AX
phosphorylation (cH2AX). Moreover, we observed an ESC-specific nuclear distribution of cH2AX after interference to cellular
processes, including histone acetylation,
您可能关注的文档
- rapid and sensitive detection of rotavirus molecular signatures using surface enhanced raman spectroscopy快速、敏感的检测轮状病毒分子签名使用表面增强拉曼光谱.pdf
- rapid analysis of saccharomyces cerevisiae genome rearrangements by multiplex ligation–dependent probe amplification快速分析重组酿酒酵母基因组的多路复用ligation-dependent探测器放大.pdf
- rapid and sensitive detection of plesiomonas shigelloides by loop-mediated isothermal amplification of the huga gene邻单胞菌属的快速、敏感的检测shigelloides huga loop-mediated等温扩增的基因.pdf
- rapid antigen detection tests for malaria diagnosis in severely ill papua new guinean children a comparative study using bayesian latent class models快速抗原检测测试疟疾诊断重症巴布新几内亚的孩子比较研究使用贝叶斯潜在的类模型.pdf
- rapid and sensitive microrna detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip快速和敏感的微rna检测层流flow-assisted树突放大优点微流控芯片上.pdf
- rapid assessment of avoidable blindness in western rwanda blindness in a postconflict setting快速评估西部可避免盲症卢旺达失明的冲突后设置.pdf
- rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection快速和有针对性的基因的基因渗入到受欢迎的小麦品种利用分子标记辅助选择背景.pdf
- rapid annotation of anonymous sequences from genome projects using semantic similarities and a weighting scheme in gene ontology匿名序列的快速注释从基因组项目使用基因本体的语义相似性和加权方案.pdf
- rapid assembly of multiple-exon cdna directly from genomic dnamultiple-exon cdna直接从基因组dna的快速组装.pdf
- rapid assessment of genetic ancestry in populations of unknown origin by genome-wide genotyping of pooled samples快速评估种群遗传祖先的来历不明的全基因组基因分型的混合样品.pdf
- redox regulation of insulin degradation by insulin-degrading enzyme氧化还原调控胰岛素降解酶降解.pdf
- rediscovery of leptoxis compacta (anthony, 1854) (gastropoda cerithioidea pleuroceridae)重新发现的leptoxis致密部(安东尼,1854)(腹足纲cerithioidea侧角螺科).pdf
- red-mediated transposition and final release of the mini-f vector of a cloned infectious herpesvirus genomered-mediated换位和最终释放mini-f向量的克隆感染疱疹病毒基因组.pdf
- redox regulation of the amp-activated protein kinase氧化还原活化蛋白激酶的调节.pdf
- redox-based inactivation of cysteine cathepsins by compounds containing the 4-aminophenol moietyredox-based半胱氨酸组织蛋白酶的失活化合物包含4-aminophenol一半.pdf
- reduced antibody responses to vaccinations in children exposed to polychlorinated biphenyls降低疫苗的抗体反应在儿童暴露于多氯联苯.pdf
- reduced basal atp synthetic flux of skeletal muscle in patients with previous acromegaly减少基底atp合成通量先前的肢端肥大症患者的骨骼肌.pdf
- reduced attentional scope in cocaine polydrug users减少在可卡因polydrug用户注意力的范围.pdf
- reduced body weight and increased energy expenditure in transgenic mice over-expressing soluble leptin receptor减少体重和增加能量消耗在转基因小鼠过度表达可溶性瘦素受体.pdf
- reduced cardiac vagal modulation impacts on cognitive performance in chronic fatigue syndrome减少心脏迷走神经在慢性疲劳综合症调制对认知能力的影响.pdf
文档评论（0）