rapid and sensitive microrna detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip快速和敏感的微rna检测层流flow-assisted树突放大优点微流控芯片上.pdfVIP

Rapid and Sensitive MicroRNA Detection with Laminar Flow-Assisted Dendritic Amplification on Power-Free Microfluidic Chip ¤ Hideyuki Arata , Hiroshi Komatsu, Kazuo Hosokawa*, Mizuo Maeda Bioengineering Laboratory, RIKEN, Wako, Japan Abstract Detection of microRNAs, small noncoding single-stranded RNAs, is one of the key topics in the new generation of cancer research because cancer in the human body can be detected or even classified by microRNA detection. This report shows rapid and sensitive microRNA detection using a power-free microfluidic device, which is driven by degassed poly(dimethylsiloxane), thus eliminating the need for an external power supply. MicroRNA is detected by sandwich hybridization, and the signal is amplified by laminar flow-assisted dendritic amplification. This method allows us to detect microRNA of specific sequences at a limit of detection of 0.5 pM from a 0.5 mL sample solution with a detection time of 20 min. Together with the advantages of self-reliance of this device, this method might contribute substantially to future point-of-care early-stage cancer diagnosis. Citation: Arata H, Komatsu H, Hosokawa K, Maeda M (2012) Rapid and Sensitive MicroRNA Detection with Laminar Flow-Assisted Dendritic Amplification on Power-Free Microfluidic Chip. PLoS ONE 7(11): e48329. doi:10.1371/journal.pone.0048329 Editor: Wei-Chun Chin, University of California, Merced, United States of America Received June 19, 2012; Accepted September 24, 2012; Published November 7, 2012 Copyright: 2012 Arata et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This wor