rapid and sensitive detection of rotavirus molecular signatures using surface enhanced raman spectroscopy快速、敏感的检测轮状病毒分子签名使用表面增强拉曼光谱.pdfVIP

Rapid and Sensitive Detection of Rotavirus Molecular Signatures Using Surface Enhanced Raman Spectroscopy 1 2 4 2 3 1 Jeremy D. Driskell , Yu Zhu , Carl D. Kirkwood , Yiping Zhao , Richard A. Dluhy , Ralph A. Tripp * 1 Department of Infectious Diseases, University of Georgia, Athens, Georgia, United States of America, 2 Department of Physics, University of Georgia, Athens, Georgia, United States of America, 3 Department of Chemistry, University of Georgia, Athens, Georgia, United States of America, 4 Enteric Virus Group, Murdoch Children’s Research Institute, The Royal Children’s Hospital, Parkville, Victoria, Australia Abstract Human enteric virus infections range from gastroenteritis to life threatening diseases such as myocarditis and aseptic meningitis. Rotavirus is one of the most common enteric agents and mortality associated with infection can be very significant in developing countries. Most enteric viruses produce diseases that are not distinct from other pathogens, and current diagnostics is limited in breadth and sensitivity required to advance virus detection schemes for disease intervention strategies. A spectroscopic assay based on surface enhanced Raman scattering (SERS) has been developed for rapid and sensitive detection of rotavirus. The SERS method relies on the fabrication of silver nanorod array substrates that are extremely SERS-active allowing for direct structural characterization of viruses. SERS spectra for eight rotavirus strains were analyzed to qualitatively identify rotaviruses and to classify each according to G and P genotype and strain with .96% accuracy, and a quantitative model based on partial least squares regression analysis was evalu