rapid and long-lasting increase in sites for synapse assembly during late-phase potentiation in rat hippocampal neurons快速和持久的增加网站在后期阶段势差在鼠海马神经元突触组装.pdfVIP

Rapid and Long-Lasting Increase in Sites for Synapse Assembly during Late-Phase Potentiation in Rat Hippocampal Neurons 1 1 1 1 1,2 Irina Antonova , Fang-Min Lu , Leonard Zablow , Hiroshi Udo , Robert D. Hawkins * 1 Department of Neuroscience, Columbia University, New York, New York, United States of America, 2 New York State Psychiatric Institute, New York, New York, United States of America Abstract Long-term potentiation in hippocampal neurons has stages that correspond to the stages of learning and memory. Early- phase (10–30 min) potentiation is accompanied by rapid increases in clusters or puncta of presynaptic and postsynaptic proteins, which depend on actin polymerization but not on protein synthesis. We have now examined changes in pre- and postsynaptic puncta and structures during glutamate-induced late-phase (3 hr) potentiation in cultured hippocampal neurons. We find that (1) the potentiation is accompanied by long-lasting maintenance of the increases in puncta, which depends on protein synthesis, (2) most of the puncta and synaptic structures are very dynamic, continually assembling and disassembling at sites that are more stable than the puncta or structures themselves, (3) the increase in presynaptic puncta appears to be due to both rapid and more gradual increases in the number of sites where the puncta may form, and also to the stabilization of existing puncta, (4) under control conditions, puncta of postsynaptic proteins behave similarly to puncta of presynaptic proteins and share sites with them, and (5) the increase in presynaptic puncta is accompanied by a similar increase in presumably presynaptic structures, which may form at distinct as well as shared sites. The ne