reduced polymorphism in domains involved in protein-protein interactions减少多态性在域参与蛋白质的相互作用.pdfVIP

Reduced Polymorphism in Domains Involved in Protein- Protein Interactions ¤ Zohar Itzhaki , Hanah Margalit* Department of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel Abstract Genome sequencing of various individuals or isolates of the same species allows studying the polymorphism level of specific proteins and protein domains. Here we ask whether domains that are known to be involved in mediating protein- protein interactions show lower polymorphism than other domains. To this end we take advantage of a recent genome sequence dataset of 39 Saccahromyces cerevisiae strains and the experimentally determined protein interaction network of the laboratory strain. We analyze the polymorphism in domain residues involved in interactions at various levels of resolution, depending on their likelihood to be interaction mediators. We find that domains involved in interactions are less polymorphic than other domains. Furthermore, as the likelihood of a residue to be involved in interaction increases, its polymorphism decreases. Our results suggest that purifying selection operates on domains capable of mediating protein interactions to maintain their function. Citation: Itzhaki Z, Margalit H (2012) Reduced Polymorphism in Domains Involved in Protein-Protein Interactions. PLoS ONE 7(4): e34503. doi:10.1371/ journal.pone.0034503 ˜ ´ Editor: Leonardo Marino-Ramırez, National Institutes of Health, United States of America Received October 24, 2011; Accepted March 2, 2012; Published April 3, 2012 Copyright: 2012 Itzhaki, Margalit. This is an open-access article distribu