regulatory effects of ifn-β on the development of experimental autoimmune uveoretinitis in b10riii mice监管的影响ifn-βb10riii小鼠实验性自身免疫性uveoretinitis的发展.pdfVIP

Regulatory Effects of IFN-b on the Development of Experimental Autoimmune Uveoretinitis in B10RIII Mice 1. 1,2. 1 1 1 3 Min Sun , Yan Yang , Peizeng Yang *, Bo Lei , Liping Du , Aize Kijlstra 1The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology and Chongqing Eye Institute, Chongqing, People’s Republic of China, 2 Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, People’s Republic of China, 3 Eye Research Institute Maastricht, Department of Ophthalmology, University Hospital Maastricht, Maastricht, The Netherlands Abstract Background: Experimental autoimmune uveoretinitis (EAU) serves as a model for human intraocular inflammation. IFN-b has been used in the treatment of certain autoimmune diseases. Earlier studies showed that it ameliorated EAU; however, the mechanisms involved in this inhibition are still largely unknown. Methodology/Principal Findings: B10RIII mice were immunized with interphotoreceptor retinoid-binding protein (IRBP) peptide 161–180 in Complete Freund’s adjuvant. Splenocytes from different time points after immunization were used to evaluate the expression of IFN-b. An increased expression of IFN-b was observed during EAU and its highest expression was observed on day 16, 3 days after the peak of intraocular inflammation. Splenocytes and draining lymph node cells from mice immunized with IRBP161-180 on day 13 and control mice were activated with anti-CD3/anti-CD28 antibodies or IRBP161-180 to evaluate the production of IFN-c and IL-17. The results showed that IFN-c and IL-17 were significantly higher in immunized mice as compared to the control mice when exposed to anti-CD3/anti-CD28 antibodies. However