reduction of canine plasminogen leads to an expanded molecule which precipitates减少宠物纤溶酶原导致扩大分子物沉淀.pdfVIP

Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates Jack A. Kornblatt* Enzyme Research Group, Department of Biology, Concordia University, Montreal, Quebec, Canada Abstract Canine plasminogen is made up of seven domains. In each domain there are several cysteines that are linked by disulfide bonds. Reduction of a limited number of the cystines destabilizes the protein such that it precipitates. The bond or bonds that are broken provide about 14 kcal of stabilization energy. Circular dichroism and dynamic light scattering indicate that there is probably an intermediate that is formed prior to precipitation and that the intermediate is somewhat larger than the compact form of plasminogen. Citation: Kornblatt JA (2009) Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates. PLoS ONE 4(7): e6196. doi:10.1371/ journal.pone.0006196 Editor: Andreas Hofmann, Griffith University, Australia Received March 19, 2009; Accepted June 13, 2009; Published July 10, 2009 Copyright: 2009 Kornblatt. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The work was supported by NSERC Canada, Grant Number 9988-06. URL: http://www.nserc.ca Competing Interests: The author has declared that no competing interests exist. * E-mail: krnbltt@alcor.concordia.ca Introduction Many blood proteins are synthesized by the liver and subsequently exported. B