reliable classifier to differentiate primary and secondary acute dengue infection based on igg elisa可靠的分类器来区分主要和次要严重的登革热感染基于免疫球蛋白g elisa.pdfVIP

Reliable Classifier to Differentiate Primary and Secondary Acute Dengue Infection Based on IgG ELISA ´ 1,2 1,4 3 Marli Tenorio Cordeiro , Ulisses Braga-Neto , Rita Maria Ribeiro Nogueira , Ernesto T. A. Marques Jr.1,5,6* ˜ 1Virology and Experimental Therapy Laboratory, Aggeu Magalhaes Research Center, Fiocruz, Recife, Pernambuco, Brazil, 2 Central Laboratory of Public Health, Secretaria ´ de Saude do Estado de Pernambuco, Recife, Brazil, 3 Laboratory of Flavivirus, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil, 4 Department of Electrical and Computer Engineering, Texas AM University, College Station, Texas, United States of America, 5 Department of Medicine, Division of Infectious Diseases, The Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America, 6 Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America Abstract Background: Dengue virus infection causes a wide spectrum of illness, ranging from sub-clinical to severe disease. Severe dengue is associated with sequential viral infections. A strict definition of primary versus secondary dengue infections requires a combination of several tests performed at different stages of the disease, which is not practical. Methods and Findings: We developed a simple method to classify dengue infections as primary or secondary based on the levels of dengue-specific IgG. A group of 109 dengue infection patients were classified as having primary or secondary dengue infection on the basis of a strict combination of results from assays of antigen-specific IgM and