remodeling of purinergic receptor-mediated ca2+ signaling as a consequence of egf-induced epithelial-mesenchymal transition in breast cancer cells重塑purinergic受体介导钙离子信号由于egf-induced epithelial-mesenchymal过渡在乳腺癌细胞.pdfVIP

Remodeling of Purinergic Receptor-Mediated Ca2+ Signaling as a Consequence of EGF-Induced Epithelial- Mesenchymal Transition in Breast Cancer Cells 1 2 3,4 3,5 3,4 Felicity M. Davis , Paraic A. Kenny , Eliza T-L Soo , Bryce J. W. van Denderen , Erik W. Thompson , 1 1 1 1 Peter J. Cabot , Marie-Odile Parat , Sarah J. Roberts-Thomson , Gregory R. Monteith * 1 School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia, 2 Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, New York, New York, United States of America, 3 St. Vincent’s Institute, Fitzroy, Victoria, Australia, 4 University of Melbourne Department Surgery, St. Vincent’s Hospital, Fitzroy, Victoria, Australia, 5 University of Melbourne Department Medicine, St. Vincent’s Hospital, Fitzroy, Victoria, Australia Abstract Background: The microenvironment plays a pivotal role in tumor cell proliferation, survival and migration. Invasive cancer cells face a new set of environmental challenges as they breach the basement membrane and colonize distant organs during the process of metastasis. Phenotypic switching, such as that which occurs during epithelial-mesenchymal transition (EMT), may be associated with a remodeling of cell surface receptors and thus altered responses to signals from the tumor microenvironment. Methodology/Principal Findings: We assessed changes in intracellular Ca2+ in cells loaded with Fluo-4 AM using a fluorometric imaging plate reader (FLIPRTETRA) and observed significant changes