remodeling of the streptococcus agalactiae transcriptome in response to growth temperature重构的链球菌agalactiae转录组,以应对增长的温度.pdfVIP

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remodeling of the streptococcus agalactiae transcriptome in response to growth temperature重构的链球菌agalactiae转录组,以应对增长的温度.pdf

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remodeling of the streptococcus agalactiae transcriptome in response to growth temperature重构的链球菌agalactiae转录组,以应对增长的温度

Remodeling of the Streptococcus agalactiae Transcriptome in Response to Growth Temperature 1,2 1 1 1 Laurent Mereghetti , Izabela Sitkiewicz , Nicole M. Green , James M. Musser * 1 Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, Houston, Texas, United States of America, ´ ´ ´ ´ 2 Universite Franc¸ois-Rabelais, Faculte de Medecine, EA3854 ‘‘Bacteries et risque materno-foetal’’ and Centre Hospitalier Universitaire, Tours, France Abstract Background: To act as a commensal bacterium and a pathogen in humans and animals, Streptococcus agalactiae (group B streptococcus, GBS) must be able to monitor and adapt to different environmental conditions. Temperature variation is a one of the most commonly encountered variables. Methodology/Principal Findings: To understand the extent to which GBS modify gene expression in response to temperatures encountered in the various hosts, we conducted a whole genome transcriptome analysis of organisms grown at 30uC and 40uC. We identified extensive transcriptome remodeling at various stages of growth, especially in the stationary phase (significant transcript changes occurred for 25% of the genes). A large proportion of genes involved in metabolism was up-regulated at 30uC in stationary phase. Conversely, genes up-regulated at 40uC relative to 30uC include those encoding virulence factors such as hemolysins and extracellular secreted proteins with LPXTG motifs. Over-expression of hemolysins was linked to larger zones of hemolysis and enhanced hemolytic activity at 40uC. A key theme identified by our study was that genes involve