re-patterning sleep architecture in drosophila through gustatory perception and nutritional quality重置睡眠架构通过味觉感知在果蝇和营养品质.pdfVIP

Re-Patterning Sleep Architecture in Drosophila through Gustatory Perception and Nutritional Quality 1 1,2 1,2 Nancy J. Linford *, Tammy P. Chan , Scott D. Pletcher 1 Department of Molecular and Integrative Physiology, Geriatrics Center and Nathan Shock Center of Excellence in the Basic Biology of Aging, University of Michigan, Ann Arbor, Michigan, United States of America, 2 Department of Human and Molecular Genetics, Huffington Center on Aging and Developmental Biology Program, Baylor College of Medicine, Houston, Texas, United States of America Abstract Organisms perceive changes in their dietary environment and enact a suite of behavioral and metabolic adaptations that can impact motivational behavior, disease resistance, and longevity. However, the precise nature and mechanism of these dietary responses is not known. We have uncovered a novel link between dietary factors and sleep behavior in Drosophila melanogaster. Dietary sugar rapidly altered sleep behavior by modulating the number of sleep episodes during both the light and dark phase of the circadian period, independent of an intact circadian rhythm and without affecting total sleep, latency to sleep, or waking activity. The effect of sugar on sleep episode number was consistent with a change in arousal threshold for waking. Dietary protein had no significant effect on sleep or wakefulness. Gustatory perception of sugar was necessary and sufficient to increase the number of sleep episodes, and this effect was blocked by activation of bitter- sensing neurons. Further addition of sugar to the diet blocked the effects of sweet gustatory perception through a gustatory-independent mechanism. However, gustatory perception was not required for diet-induced fat accumulation, indicati