repeated abortion affects subsequent pregnancy outcomes in balbc mice反复流产影响随后的妊娠结果balbc老鼠.pdfVIP

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repeated abortion affects subsequent pregnancy outcomes in balbc mice反复流产影响随后的妊娠结果balbc老鼠.pdf

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repeated abortion affects subsequent pregnancy outcomes in balbc mice反复流产影响随后的妊娠结果balbc老鼠

Repeated Abortion Affects Subsequent Pregnancy Outcomes in BALB/c Mice 1,2 2 2 1,2 2 2 1,2 Fang Lv , Xiangbo Xu , Shucheng Zhang , Lili Wang , Ning Wang , Bin He *, Jiedong Wang * 1 Graduate School, Peking Union Medical College, Beijing, China, 2 Reproductive Physiology Laboratory, National Research Institute for Family Planning, Beijing, China Abstract Aim: In this study, we aimed to establish a mouse model of repeated medical termination of pregnancy in order to determine subsequent outcomes. Methods: A model of mifepristone (RU 486)-induced medical abortion was established in BALB/c mice to facilitate the investigation of the impact of medical abortion on subsequent pregnancies, including litter sizes and newborn birth weights. Pregnant mice were sacrificed to examine midterm pregnancy status, investigate the frequency of fetal resorption, and measure placental function gene expression by real-time PCR and immunohistochemistry. Offspring liver mRNA was harvested for real-time PCR to determine gene expression and assess the effects of abortion on offspring development. Results: Mice subjected to 2 previous medical abortions experienced spontaneous abortions in subsequent pregnancies. Medical abortion caused reduced reproductive capacity and affected placental dysfunction, with reduced expression of tissue factor (TF) and genes encoding proteins involved in metabolic functions relevant to pregnancy, such as 11b- hydroxysteroid dehydrogenase 1/2 (11b-HSD1/2) and glucocorticoid receptor (GR). Reduced expression was also observed for platelet endothelial cell adhesion molecule-1 (CD31) and vascular endothelial growth