role of plastid transglutaminase in lhcii polyamination and thylakoid electron and proton flow质体转谷氨酰胺酶的作用在lhcii polyamination和类囊体电子和质子流.pdfVIP

Role of Plastid Transglutaminase in LHCII Polyamination and Thylakoid Electron and Proton Flow 1,2 2 2. ´ 2. 1 . Nikolaos E. Ioannidis , Oriol Lopera , Mireya Santos , Josep M. Torne , Kiriakos Kotzabasis * ` 1 Department of Biology, University of Crete, Voutes University Campus, Heraklion, Crete, Greece, 2 Departament de Genetica Molecular, Centre for Research in Agricultural Genomics, CRAG (CSIC-IRTA-UAB-UB), Barcelona, Spain Abstract Transglutaminases function as biological glues in animal cells, plant cells and microbes. In energy producing organelles such as chloroplasts the presence of transglutaminases was recently confirmed. Furthermore, a plastidial transglutaminase has been cloned from maize and the first plants overexpressing tgz are available (Nicotiana tabacum TGZ OE). Our hypothesis is that the overexpression of plastidal transglutaminase will alter photosynthesis via increased polyamination of the antenna of photosystem II. We have used standard analytical tools to separate the antenna from photosystem II in wild type and modified plants, 6 specific antibodies against LHCbs to confirm their presence and sensitive HPLC method to quantify the polyamination level of these proteins. We report that bound spermidine and spermine were significantly increased (,80%) in overexpressors. Moreover, we used recent advances in in vivo probing to study simultaneously the proton and electron circuit of thylakoids. Under physiological conditions overexpressors show a 3-fold higher sensitivity of the antenna down regulation loop (qE) to the elicito