原创力文档riboflavinuva collagen cross-linking-induced changes in normal and keratoconus corneal stromariboflavinuva胶原cross-linking-induced正常和圆锥形角膜角膜基质的变化.pdfVIP
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riboflavinuva collagen cross-linking-induced changes in normal and keratoconus corneal stromariboflavinuva胶原cross-linking-induced正常和圆锥形角膜角膜基质的变化
Riboflavin/UVA Collagen Cross-Linking-Induced Changes
in Normal and Keratoconus Corneal Stroma
1 1 1 2 1
Sally Hayes , Craig Boote , Christina S. Kamma-Lorger , Madhavan S. Rajan , Jonathan Harris , Erin
1 3 4 4 5 6
Dooley , Nicholas Hawksworth , Jennifer Hiller , Nick J. Terill , Farhad Hafezi , Arun K. Brahma ,
1 1
Andrew J. Quantock , Keith M. Meek *
1 Structural Biophysics Research Group, School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom, 2 Vision and Eye Research Unit, Anglia
Ruskin University, Cambridge, United Kingdom, 3 Royal Glamorgan Hospital, Llantrisant, Rhondda Cynon Taff, United Kingdom, 4 Diamond Light Source, Didcot,
Oxfordshire, United Kingdom, 5 Division of Ophthalmology, Geneva University Hospitals, Geneva, Switzerland, 6 Manchester Royal Eye Hospital, Manchester, United
Kingdom
Abstract
Purpose: To determine the effect of Ultraviolet-A collagen cross-linking with hypo-osmolar and iso-osmolar riboflavin
solutions on stromal collagen ultrastructure in normal and keratoconus ex vivo human corneas.
Methods: Using small-angle X-ray scattering, measurements of collagen D-periodicity, fibril diameter and interfibrillar
spacing were made at 1 mm intervals across six normal post-mortem corneas (two above physiological hydration (swollen)
and four below (unswollen)) and two post-transplant keratoconus corneal buttons (one swollen; one unswollen), before and
after hypo-osmolar cross-linking. The same parameters were measured in three other unswollen
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